Recombinant Human Apolipoprotein A-I 在大腸桿菌 (E. coli) 中表達。Apolipoprotein A-I (ApoA-I) 是高密度脂蛋白 (HDL) 的主要蛋白成分,在膽固醇反向轉運途徑中起關鍵作用。Apolipoprotein A-I (ApoA-I) 與登革熱病毒 (DV) 顆粒相關,并通過 SR-BI 增強病毒感染。 Synonyms rHuApolipoprotein A-I; ApoA1; Apolipoprotein A-I Species HumanSource E. coli Accession P02647 Gene ID 335 Molecular Weight 27-29 kDa AA Sequence RHFWQQDEPP QSPWDRVKDL ATVYVDVLKD SGRDYVSQFE GSALGKQLNL KLLDNWDSVT STFSKLREQL GPVTQEFWDN LEKETEGLRQ EMSKDLEEVK AKVQPYLDDF QKKWQEEMEL YRQKVEPLRA ELQEGARQKL HELQEKLSPL GEEMRDRARA HVDALRTHLA PYSDELRQRL AARLEALKEN GGARLAEYHA KATEHLSTLS EKAKPALEDL RQGLLPVLES FKVSFLSALE EYTKKLNTQ Biological Activity Data is not available. Appearance Lyophilized powder. Formulation Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.4. Endotoxin Level <1 EU/μg, determined by LAL method. Reconstitution Reconstitute the lyophilized recombinant Human Apolipoprotein A-I (rHuApolipoprotein A-I) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability Lyophilized recombinant Human Apolipoprotein A-I (rHuApolipoprotein A-I) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping Room temperature in continental US; may vary elsewhere. Background Apolipoprotein A1 (ApoA1) is a 28 kDa glycoprotein that is the major protein component of high density lipoprotein (HDL) particles. HDL particles play a central role in the reverse transport of cholesterol from peripheral tissues to the liver. In the past decade, reconstituted HDL (rHDL) has been employed as a therapeutic agent for treatment of atherosclerosis. The ability of rHDL to promote cholesterol efflux from peripheral cells has been documented to reduce the size of atherosclerotic plaque lesions[2]. |